​The law applies to all animals used for the purposes of basic research, higher education and training: vertebrates and their independently feeding larvae, foetuses of mammalian species in their last trimester of development as well as cephalopods.

The main principle is that an animal experiment is not allowed to be conducted without a license from the Animal Experiment Board (project license) and the person who performs the animal experiments must be competent.
Live animals cannot be used in studies where the same results can be obtained by other methods.
It must be ensured that only the minimum number of animals is used for scientific or educational purposes and that minimal pain, suffering, distress or lasting harm is caused to the animals.